Atom Ant (originally voiced by Howard Morris, then by Don Messick in later episodes) is a superhero ant who operated out of an anthill in the countryside, where he possessed such things as a mainframe computer and exercise equipment. His powers mostly consisted of the ability to fly, superspeed, incredible strength, and invulnerability. His catchphrase was "Up and at 'em, Atom Ant!" He was often contacted by the police, who sent him out on an assignment.
Some of these missions parodied the missions of Batman. The police force was constantly shown to be underfunded and inept, as they relied on Atom Ant to do all their police work. As seen in "Nobody's Fool," the only two police officers were the chief of police and deputy chief. The department only possessed one rusted patrol car. Atom Ant fights various villains including recurring ones like Ferocious Flea (also voiced by Messick) and mad scientist Professor Von Gimmick.