Following the plot of the Carmen Sandiego franchise, Earth sees international thief Carmen Sandiego lead the organization V.I.L.E. in stealing treasures from around the world and leaving clues behind for ACME agents Zack (voiced by Scott Menville) and Ivy (voiced by Jennifer Hale), under the guidance of the Chief (voiced by Rodger Bumpass), to find in order to capture her. In this version, Carmen Sandiego is a former agent of ACME who left when she sought a greater challenge and has a strong code of ethics when stealing items. The Player is a live-action unseen character who bookends acts by communicating with Carmen; it is implied that to them the television series is a video game that they are playing from a computer.
While Carmen is originally presented as the show's antagonist, she becomes more like an anti-hero as the series progressed where she even helped Zack and Ivy against mutual enemies